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Related Experiment Videos

Complement activation and lung permeability during cardiopulmonary bypass.

S D Tennenberg1, C W Clardy, W W Bailey

  • 1Department of Surgery, University of Cincinnati College of Medicine, Ohio.

The Annals of Thoracic Surgery
|October 1, 1990
PubMed
Summary

Cardiopulmonary bypass activates complement and neutrophils, but does not cause acute lung injury. This suggests other factors may cause lung permeability issues in sepsis and trauma.

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Area of Science:

  • Cardiovascular Surgery
  • Pulmonary Medicine
  • Immunology

Background:

  • Pulmonary dysfunction post-cardiopulmonary bypass (CPB) is linked to complement activation.
  • Understanding the mechanisms of lung injury during CPB is crucial.

Purpose of the Study:

  • To investigate the relationship between complement activation, neutrophil response, and pulmonary epithelial permeability during CPB.
  • To determine if CPB-induced complement and neutrophil activation leads to acute lung injury.

Main Methods:

  • Measured plasma levels of activated complement components (C3adesArg, C4adesArg, C5adesArg) using radioimmunoassay.
  • Assessed neutrophil n-formyl-methionyl-leucyl-phenylalanine (FMLP) receptor expression via radioligand binding assays.
  • Quantified pulmonary epithelial permeability using radioaerosol lung clearance of 99mTc-DTPA.

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Main Results:

  • Significant elevations in plasma C3adesArg, C4adesArg, and C5adesArg were observed post-CPB.
  • Increased neutrophil FMLP receptor expression indicated neutrophil activation.
  • No significant increase in pulmonary epithelial permeability was detected despite complement and neutrophil activation.

Conclusions:

  • Complement and neutrophil activation during CPB are not directly associated with acute pulmonary epithelial injury.
  • Findings suggest that factors other than complement and neutrophil activation may contribute to lung permeability in sepsis- and trauma-induced ARDS.