Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Determination of Crystal Structures01:29

Determination of Crystal Structures

In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sentinel Chickens and Their Role in Mosquito-Borne Virus Surveillance in Delaware.

Delaware journal of public health·2021
Same author

A Singular Case of an Eruptive Disease.

The Medical and physical journal·2018
Same author

An Inveterate Case of Tinea Capitis.

The Medical and physical journal·2018
Same author

What Can We Learn from Wide-Angle Solution Scattering?

Advances in experimental medicine and biology·2017
Same author

Effects of Catalytic Action and Ligand Binding on Conformational Ensembles of Adenylate Kinase.

Biochemistry·2017
Same author

Rice Cellulose SynthaseA8 Plant-Conserved Region Is a Coiled-Coil at the Catalytic Core Entrance.

Plant physiology·2016

Related Experiment Video

Updated: May 26, 2026

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin
06:29

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Published on: March 3, 2021

Crystallographic fragment screening.

John Badger1

  • 1Zenobia Therapeutics, San Diego, CA, USA. info1.dgtech@gmail.com

Methods in Molecular Biology (Clifton, N.J.)
|January 7, 2012
PubMed
Summary

Crystallographic fragment screening uses X-ray diffraction to identify potential drug fragments that bind to protein targets. Designing effective fragment libraries is key for successful drug discovery initiatives.

Area of Science:

  • Structural biology
  • Drug discovery
  • Biophysics

Background:

  • Crystallographic fragment screening is a vital technique for initiating drug discovery.
  • It involves analyzing X-ray diffraction data to visualize small molecule binding to protein crystals.

Purpose of the Study:

  • To outline the methodology and challenges of crystallographic fragment screening.
  • To emphasize the importance of appropriate fragment library design for successful drug discovery.

Main Methods:

  • Soaking or growing protein crystals with small molecule compounds (110-250 Da).
  • Analyzing X-ray diffraction data to visualize compound binding in electron density maps.
  • Utilizing high-throughput structure determination for efficient data analysis.

More Related Videos

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
08:35

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

Published on: May 29, 2021

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
06:17

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

Published on: February 28, 2025

Related Experiment Videos

Last Updated: May 26, 2026

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin
06:29

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Published on: March 3, 2021

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
08:35

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

Published on: May 29, 2021

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
06:17

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

Published on: February 28, 2025

Main Results:

  • A diverse library of a few hundred compounds can comprehensively screen protein targets.
  • Fragment screening can be completed with approximately 100 diffraction datasets.
  • Designing or procuring suitable fragment libraries is a primary practical challenge.

Conclusions:

  • Successful crystallographic fragment screening relies on well-designed fragment libraries.
  • High-throughput methods and robotic sample handling are crucial for efficiency.
  • Informed compound selection and data analysis are essential for drug discovery.