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Related Experiment Video

Updated: May 26, 2026

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
09:53

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

Whole-mount confocal microscopy for vascular branching morphogenesis.

Yoh-suke Mukouyama1, Jennifer James, Joseph Nam

  • 1Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. mukoyamay@mail.nih.gov

Methods in Molecular Biology (Clifton, N.J.)
|January 7, 2012
PubMed
Summary

We developed a new whole-mount immunohistochemistry technique to study blood and lymphatic vessel development in mouse embryos. This method uses advanced microscopy for high-resolution imaging of complex vascular networks.

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Related Experiment Videos

Last Updated: May 26, 2026

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
09:53

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

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Published on: January 14, 2021

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Published on: May 23, 2025

Area of Science:

  • Developmental Biology
  • Vascular Biology
  • Immunohistochemistry

Background:

  • Understanding vascular network formation is crucial for developmental biology.
  • Existing methods may have limitations in visualizing complex branching patterns.

Purpose of the Study:

  • To present a novel whole-mount immunohistochemistry technique.
  • To enable detailed analysis of vascular branching morphogenesis in embryonic tissues.

Main Methods:

  • Whole-mount immunohistochemistry on mouse embryonic tissues.
  • Laser scanning confocal microscopy with multiple fluorescent labeling.
  • High-resolution imaging of intricate vascular networks.

Main Results:

  • Successful visualization of blood and lymphatic vessel branching.
  • Demonstration of robust imaging with excellent resolution.
  • Detailed analysis of complex vascular network formation.

Conclusions:

  • The developed method provides a powerful tool for studying vascular development.
  • This technique offers high-resolution insights into branching morphogenesis.
  • It facilitates comprehensive analysis of embryonic vascular networks.