Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A NIR fluorotag reporter CETIF6a enables bright pan-tumor labeling and functional proteomic profiling.

Nature communications·2026
Same author

Ligament of anterior horn of medial meniscus of knee joint: case reports and literature review.

BMC musculoskeletal disorders·2026
Same author

Deciphering the cluster-to-spinel transformation pathway of CoMn<sub>2</sub> precursors toward efficient bifunctional electrocatalysis.

Journal of colloid and interface science·2026
Same author

Late infection of thoracic aortic stent graft with aerodigestive fistula: a case series and narrative literature review.

Frontiers in cardiovascular medicine·2026
Same author

Postoperative Length of Stay After Minimally Invasive Transforaminal Lumbar Interbody Fusion: Analysis of Perioperative Predictors.

Journal of visualized experiments : JoVE·2026
Same author

A Novel Na<sub>15</sub>Sn<sub>4</sub>/NaI Biphasic Interface Layer: Synergistic Regulation of Sodium Deposition and Interface Stability for Superior Sodium Metal Anodes.

Small (Weinheim an der Bergstrasse, Germany)·2026

Related Experiment Video

Updated: May 26, 2026

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis
06:05

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

Published on: May 25, 2017

Visualization of mouse embryo angiogenesis by fluorescence-based staining.

Yang Liu1, Marc Antonyak, Xu Peng

  • 1Department of Systems Biology and Translational Medicine, College of Medicine, Texas A&M Healthy Science Center, Temple, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 7, 2012
PubMed
Summary

This study details using PECAM-1 staining to detect blood vessels in mouse embryos. This method aids in understanding embryonic vascular development and related diseases.

More Related Videos

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
08:47

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo

Published on: July 9, 2013

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound
10:39

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

Published on: March 4, 2015

Related Experiment Videos

Last Updated: May 26, 2026

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis
06:05

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

Published on: May 25, 2017

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
08:47

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo

Published on: July 9, 2013

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound
10:39

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

Published on: March 4, 2015

Area of Science:

  • Embryology
  • Vascular Biology
  • Cell Biology

Background:

  • Blood vessel network formation is crucial for embryonic development.
  • Vascular endothelial cells are key to blood vessel formation and integrity.
  • Dysfunctional blood vessels are implicated in diseases like cancer and heart disease.

Purpose of the Study:

  • To describe the utility of PECAM-1 staining for visualizing blood vessels.
  • To demonstrate PECAM-1 as a reliable marker for endothelial cells in mouse embryos.
  • To provide a method for studying embryonic vascularization.

Main Methods:

  • PECAM-1 staining technique.
  • Application to whole-mount mouse embryo tissues.
  • Application to sectioned mouse embryo tissues.

Main Results:

  • Successful visualization of the blood vessel network in mouse embryos using PECAM-1.
  • PECAM-1 staining confirmed its expression in all endothelial cells.
  • Detailed imaging of embryonic vasculature was achieved.

Conclusions:

  • PECAM-1 is an effective marker for identifying endothelial cells and mapping blood vessels in embryonic development.
  • The described staining methods are valuable tools for research in vascular biology and developmental studies.
  • Understanding embryonic vascularization is critical for addressing diseases associated with blood vessel abnormalities.