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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Aptasensor for ampicillin using gold nanoparticle based dual fluorescence-colorimetric methods.

Kyung-Mi Song¹, Euiyoung Jeong, Weejeong Jeon

¹Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyungbuk, South Korea.

Analytical and Bioanalytical Chemistry

|January 7, 2012

Summary

This summary is machine-generated.

A novel aptasensor detects ampicillin using dual fluorescence and colorimetry. This method offers high sensitivity for antibiotic detection in food products like milk.

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Area of Science:

Biosensing
Nanotechnology
Analytical Chemistry

Background:

Ampicillin is a widely used antibiotic.
Accurate detection of ampicillin residues in food is crucial for public health.
Existing detection methods may lack sensitivity or require complex procedures.

Purpose of the Study:

To develop a sensitive and specific aptasensor for ampicillin detection.
To create a dual-mode detection system combining fluorescence and colorimetry.
To validate the aptasensor for detecting ampicillin in food matrices.

Main Methods:

Selection of single-stranded DNA (ssDNA) aptamers against ampicillin using magnetic bead-based SELEX.
Development of a gold nanoparticle-based dual fluorescence-colorimetric aptasensor.
Utilizing a 5'-fluorescein amidite (FAM)-modified aptamer as a dual probe.

Main Results:

Selected aptamers (AMP4, AMP17, AMP18) demonstrated high specificity and affinity for ampicillin (K(d) values in nM range).
The dual aptasensor achieved low limits of detection: 2 ng/mL (fluorescence) and 10 ng/mL (colorimetry).
Detection limits were below the maximum residue limit for ampicillin in milk and distilled water.

Conclusions:

The developed aptasensor is highly sensitive and specific for ampicillin detection.
The dual fluorescence-colorimetric approach provides accurate antibiotic detection in food products.
This method is suitable for monitoring ampicillin residues in milk and animal tissues.