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An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization
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Cryosurgery of the inferior nasal turbinates

E Chiossone1, J R Gutierrez, J L Emmanuelli

  • 1Venezuelan Foundation of Otology, Caracas.

Auris, Nasus, Larynx
|January 1, 1990
PubMed

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...