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Updated: May 26, 2026
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High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
Flow injection-chemiluminescence determination of acyclovir
1School of Chemistry and Chemical Engineering, Southwest University, Chongqing, People's Republic of China. long0509@swu.edu.cn
Summary
A new chemiluminescence method determines acyclovir (ACV) using potassium permanganate and formaldehyde. This rapid and simple technique accurately quantifies ACV in pharmaceuticals.
Area of Science:
- Analytical Chemistry
- Chemical Analysis
Background:
- Acyclovir (ACV) is a crucial antiviral medication.
- Accurate quantification of ACV in pharmaceutical formulations is essential for quality control.
Purpose of the Study:
- To develop and validate a new chemiluminescence (CL) method for acyclovir (ACV) determination.
- To apply the developed method for the analysis of ACV in pharmaceutical samples.
Main Methods:
- Investigation of the chemiluminescence reaction between acyclovir and potassium permanganate with formaldehyde as an enhancer.
- Utilizing a flow-injection system for rapid analysis.
- Establishing the linear range, limit of detection, and precision of the method.
Main Results:
- The method demonstrated a wide linear range for ACV determination (0.2–80 mg/L).
- A low limit of detection (0.06 mg/L) and good precision (RSD of 3.7% at 1.0 mg/L) were achieved.
- The method was successfully applied to determine ACV in pharmaceutical samples with satisfactory results.
Conclusions:
- A novel, rapid, effective, and simple chemiluminescence method for acyclovir determination has been established.
- The developed flow-injection chemiluminescence method is suitable for the quality control of acyclovir in pharmaceutical products.
