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Simultaneous purification and site-specific modification of pyrroline-carboxy-lysine proteins
Hsien-Po Chiu1, Jan Grünewald, Xueshi Hao
1Genomics Institute of the Novartis Research Foundation, 10675 John-Jay-Hopkins Drive, San Diego, CA 92121, USA.
Chembiochem : a European Journal of Chemical Biology
|January 7, 2012
Abstract:
Sticky residue: Pyrroline-carboxy-lysine (Pcl) can be readily incorporated into proteins expressed in E. coli and mammalian cells by using the pyrrolysyl tRNA/tRNA synthetase pair. Pcl can be used as a single amino acid purification tag and can be site-specifically modified with functional probes during the elution process.
