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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.

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Related Experiment Video

Updated: May 26, 2026

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F&#8722;
06:53

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−

Published on: July 27, 2018

Subparticle ultrafast spectrum imaging in 4D electron microscopy.

Aycan Yurtsever1, Renske M van der Veen, Ahmed H Zewail

  • 1Physical Biology Center for Ultrafast Science and Technology, Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, CA 91125, USA.

Science (New York, N.Y.)
|January 7, 2012
PubMed
Summary

Researchers developed subparticle imaging, achieving nanometer spatial, femtosecond temporal, and millielectron volt energy resolution. This technique maps nanoscale dielectric fields, enhancing elemental analysis and plasmonics studies.

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Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures
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Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Published on: October 9, 2012

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Last Updated: May 26, 2026

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F&#8722;
06:53

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−

Published on: July 27, 2018

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures
08:53

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Published on: October 9, 2012

Area of Science:

  • Nanoscience
  • Molecular Biology
  • Cell Biology
  • Materials Science

Background:

  • Single-particle imaging is crucial for nanoscience and biological studies.
  • Existing techniques often face limitations in simultaneous space, time, and energy resolution.

Purpose of the Study:

  • To develop a subparticle imaging technique with enhanced spatial, temporal, and energy resolutions.
  • To enable simultaneous mapping of energy-time and space-time dynamics at the nanoscale.

Main Methods:

  • Utilized scanning electron probes across optically excited nanoparticles and interfaces.
  • Simultaneously constructed energy-time and space-time maps.
  • Obtained spectrum images for nanoscale dielectric fields, with photon-limited energy resolution.

Main Results:

  • Achieved spatial resolution of nanometers, temporal resolution of femtoseconds, and energy resolution of millielectron volts.
  • Demonstrated the technique on silver nanoparticles and a copper-vacuum interface.
  • Successfully mapped nanoscale dielectric fields with high energy resolution.

Conclusions:

  • The developed subparticle imaging combines electron microscopy's spatial resolution with optical techniques' energy resolution and ultrafast response.
  • This advancement opens new avenues for elemental analysis, interface mapping, and plasmonics research.