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Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70
Published on: August 9, 2019
Multiple factors influence glomerular albumin permeability in rats
Ruben M Sandoval1, Mark C Wagner, Monica Patel
1Division of Nephrology, Department of Medicine, Indiana Center for Biological Microscopy, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Journal of the American Society of Nephrology : JASN
|January 7, 2012
Summary
Investigating albumin filtration, this study identified key factors influencing the glomerular sieving coefficient (GSC(A)). Standardizing methods is crucial for accurate albumin quantification and understanding kidney permeability.
Area of Science:
- Nephrology
- Physiology
- Microscopy
Background:
- Conflicting results exist regarding albumin filtration quantification using two-photon microscopy.
- Standard operating procedures for determining the glomerular sieving coefficient for albumin (GSC(A)) are needed.
Purpose of the Study:
- Investigate factors influencing GSC(A) to explain discordant reports.
- Develop standardized methods for GSC(A) determination.
Main Methods:
- Two-photon microscopy was used to quantify albumin filtration.
- Factors analyzed included imaging depth, fluorophore selection, plasma region measurement, dextran characteristics, dietary status, and rat strain.
- Albumin transcytosis was documented in S1 proximal tubule cells.
Main Results:
- GSC(A) was influenced by imaging depth (10-20 μm optimal), longer wavelength fluorophores, plasma measurement regions, dextran properties, diet, and rat strain.
- Fasting significantly reduced GSC(A) in Simonsen Munich Wistar rats (0.035±0.005 to 0.016±0.004).
- Frömter Munich Wistar rats exhibited lower GSC(A) in both fed and fasted states.
Conclusions:
- Identified critical factors affecting GSC(A) measurements, explaining previous discrepancies.
- Highlighted the dynamic nature of glomerular albumin permeability, including extensive transcytosis.
- Provides a basis for standardized protocols in albumin filtration studies.
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