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Chromosomal assignment of the human homologue encoding SGP-2
K Slawin1, I S Sawczuk, C A Olsson
1Department of Urology, Columbia University College of Physicians and Surgeons, New York, NY 10032.
Biochemical and Biophysical Research Communications
|October 15, 1990
Summary
Researchers identified the human gene for sulfated glycoprotein-2 (SGP-2), a protein rapidly induced during apoptosis. This crucial gene responsible for programmed cell death was located on human chromosome 8.
Area of Science:
- Genetics
- Cell Biology
- Molecular Biology
Background:
- Sulfated glycoprotein-2 (SGP-2) is known for its rapid induction during apoptosis (programmed cell death).
- Its initial characterization identified it as a constitutively expressed gene product in mammalian Sertoli cells.
Purpose of the Study:
- To determine the chromosomal location of the human homologue of SGP-2.
- To map the gene responsible for SGP-2 expression in humans.
Main Methods:
- Southern blot analysis was performed on Bgl II restricted genomic DNA.
- DNA was extracted from a panel of cloned hamster-human hybrid cell lines.
- Screening for restriction fragments homologous to SGP-2 was conducted.
Main Results:
- The human homologue of SGP-2 was successfully identified through DNA analysis.
- The gene for SGP-2 was mapped to a specific human chromosome.
Conclusions:
- The human homologue of sulfated glycoprotein-2 (SGP-2) resides on chromosome 8.
- This finding provides a key genetic marker for SGP-2 and its role in apoptosis.