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Bioreactor Design and Operational System

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...

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Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models

Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models

Published on: August 9, 2012

Computational simulation modelling of bioreactor configurations for regenerating human bladder.

Seokwon Pok¹, Dhananjay V Dhane, Sundararajan V Madihally

¹a School of Chemical Engineering, Oklahoma State University , 423 Engineering North, Stillwater , OK 74078 , USA.

Computer Methods in Biomechanics and Biomedical Engineering

|January 10, 2012

Summary

This study optimized a bioreactor for human bladder regeneration, finding Design 2 with specific scaffold placement best for uniform nutrient distribution and cell growth. Increased flow rates are crucial for maintaining permeability with high smooth muscle cell densities.

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Published on: December 10, 2020

Area of Science:

Biomedical Engineering
Tissue Engineering
Regenerative Medicine

Background:

Human bladder regeneration requires advanced bioreactor systems.
Scaffold design and fluid dynamics are critical for successful tissue engineering.
Optimizing nutrient delivery and waste removal is essential for cell viability and proliferation.

Purpose of the Study:

To investigate and computationally simulate a bioreactor design for human bladder regeneration.
To evaluate the impact of different bioreactor configurations and flow rates on shear stress and nutrient distribution.
To determine the optimal bioreactor design for supporting high smooth muscle cell densities.

Main Methods:

Computational fluid dynamics (CFD) simulations were employed.
Brinkman equation, Michaelis-Menten kinetics, and Mackie-Meares relationship were utilized.
Simulations assessed various inlet designs, scaffold placements, and flow rates (0.5-5 ml/min).

Main Results:

Design 2, featuring an expanded inlet, demonstrated superior uniform shear stress and nutrient distribution.
Scaffold placement in Designs 2-A and 2-C facilitated diffusion-dominant flow mechanisms.
The bioreactor design supports increased smooth muscle cell densities, but higher flow rates are needed to counteract reduced permeability.

Conclusions:

The investigated bioreactor design shows promise for human bladder regeneration.
Optimized shear stress and nutrient distribution are achievable with specific bioreactor configurations.
Further optimization of flow rates is necessary to accommodate high cell densities in engineered bladder tissues.