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Related Experiment Video

Updated: May 26, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Trends in ultrasensitive proteomics.

A F Maarten Altelaar1, Albert J R Heck

  • 1Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.

Current Opinion in Chemical Biology
|January 10, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Recent advances in sample preparation, chromatography, and mass spectrometry enable ultra-sensitive protein analysis. This high-sensitivity proteomics is crucial for studying single cells, organelles, stem cells, and small tissue populations.

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Global protein analysis requires highly sensitive techniques.
  • Advancements in analytical instrumentation are rapidly evolving.
  • Understanding cellular and tissue composition necessitates precise protein quantification.

Purpose of the Study:

  • To review recent developments in techniques for ultra-sensitive global protein analysis.
  • To highlight the expanding applications of highly sensitive mass spectrometry-based proteomics.
  • To discuss the relevance of these advancements for specific biological samples.

Main Methods:

  • Review of recent literature on sample preparation techniques.
  • Analysis of trends in pre-fractionation and chromatography methods.

Related Experiment Videos

Last Updated: May 26, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

  • Evaluation of advancements in mass spectrometry for proteomics.
  • Focus on ultra-sensitive global analysis of proteins.

    • Main Results:

    • Significant progress has been made in sample preparation and pre-fractionation.
    • Chromatography and mass spectrometry technologies have achieved unprecedented sensitivity.
    • These advancements facilitate the proteome analysis of challenging biological samples.

    Conclusions:

    • Ultra-sensitive mass spectrometry-based proteomics is becoming a reality for single-cell analysis.
    • The reviewed techniques are highly relevant for studying subcellular organelles.
    • These methods are critical for analyzing low-abundant cell types like stem cells and small, homogeneous cell populations from tissues.