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A Semi-Automated Workflow for the Cryopreservation of Coral Sperm to Support Biobanking and Aquaculture

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Published on: June 7, 2024

Palm cryobanking.

Lotfi Fki¹, Neila Bouaziz, Nahed Sahnoun

¹Laboratory of Plant Biotechnology, Faculty of Sciences, University of Sfax, Sfax, Tunisia. lotfifki@yahoo.fr

|January 10, 2012

Summary

An efficient cryopreservation protocol using ultra-rapid droplet-vitrification was developed for date palm (Phoenix dactylifera) proembryogenic masses. This method ensures true-to-type plant regeneration and maintains genetic stability for date palm conservation.

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Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells

Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells

Published on: October 20, 2023

Area of Science:

Plant Biotechnology
Cryobiology
Horticulture

Background:

Date palm (Phoenix dactylifera) propagation relies on efficient in vitro techniques.
Cryopreservation is crucial for long-term conservation of valuable date palm genotypes.
Developing robust protocols for date palm proembryogenic masses (PEMs) is essential.

Purpose of the Study:

To develop an efficient cryopreservation protocol for 'Barhee' date palm PEMs.
To evaluate the ultra-rapid droplet-vitrification technique against standard vitrification.
To assess the impact of cryopreservation on the genetic stability and regeneration capacity of date palms.

Main Methods:

Induction of PEMs from juvenile leaves on MS medium with 2,4-D.
Application of ultra-rapid droplet-vitrification with PVS2 treatment at varying temperatures and durations.
Sucrose preculture and assessment of post-cryopreservation recovery and regeneration rates.
Establishment of suspension cultures and somatic embryo production from cryopreserved PEMs.

Main Results:

Ultra-rapid droplet-vitrification was superior to standard vitrification for date palm PEMs.
Sucrose preculture significantly enhanced post-cryopreservation recovery.
Regeneration rates reached up to 63.3% with optimized PVS2 treatment.
Cryopreserved PEMs showed high proliferation and somatic embryo production (1030 +/- 50 units/500 mg).

Conclusions:

The developed ultra-rapid droplet-vitrification protocol is efficient for date palm cryopreservation.
Cryopreservation maintained the morphogenetic capacity and genetic stability of 'Barhee' date palm.
This protocol facilitates large-scale conservation and propagation of elite date palm varieties.