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Seminal freezing in pure breed andalusian horse: difference in individual stallions and correlation between pre and

L Ruiz1, A Echegaray, A Lafuente

  • 1C.I.F.E.A. of Lorca (Murcia). Consejería de Agricultura y Agua, Murcia, Spain.

Cryo Letters
|January 10, 2012
PubMed

The aim of this study was the optimization of the sperm freezing protocols for the Pure Breed Andalusian Horse (AH) stallions. The study was performed in 84 ejaculates from 14 stallions (6 ejaculates per stallion). We examined the effect of individual stallion, centrifugal force and centrifugation extender on post-thaw sperm quality. Neither centrifugal force nor centrifugal extender had any significant effect on post-centrifugation or post-thawing sperm quality. Stallion was the principal source of variation in our experiments, showing individual significant differences (p < 0.05) in all parameters. Individual differences were more extreme prior to freezing than after freezing-thawing. There are significant positive correlations (p < 0.05 and p < 0.01) between all post-centrifugation and post-thawing parameters.

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