Related Experiment Video
Updated: May 26, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Complete genome sequence of a potyvirus infecting yam beans (Pachyrhizus spp.) in Peru
Segundo Fuentes1, Bettina Heider, Ruby Carolina Tasso
1International Potato Center, Apartado, 1558, Lima 12, Peru.
Abstract:
In 2010, yam beans in a field trial in Peru showed viral disease symptoms. Graft-transmission and positive ELISA results using potyvirus-specific antibodies suggested that the symptoms could be the result of a potyviral infection. Small interfering RNA (siRNA) were extracted from one of the samples and sent for high-throughput sequencing. The full genome of a new potyvirus could be assembled from the resulting siRNA sequences, and it was sufficiently different from other sequences to be considered a member of a new species, which we have designated Yam bean mosaic virus (YBMV). Sequence similarity suggests that YBMV has also been detected in yam beans in Indonesia.
More Related Videos
Related Concept Videos
Subviral Agents
Viruses with RNA Genomes

