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Published on: May 10, 2019
Identification and characterization of a noncoding RNA at the mouse Pcna locus
Ji-Yeon Lee1, Abdul Aziz Khan, Hyehyun Min
1Department of Anatomy, Embryology Laboratory, Yonsei University College of Medicine, Seoul, 120-752, Korea.
Molecules and Cells
|January 10, 2012
Summary
A novel noncoding RNA (ncRNA) positively regulates the neighboring Pcna gene in normal cells. This regulatory function is disrupted in cancer cells, impacting Pcna gene expression.
Area of Science:
- Molecular Biology
- Gene Regulation
- Cancer Biology
Background:
- AK007836 is a 2-exon noncoding RNA (ncRNA) located upstream of the Pcna gene.
- Pcna (Proliferating Cell Nuclear Antigen) is a crucial protein involved in DNA replication and repair.
Purpose of the Study:
- To investigate the expression pattern and regulatory role of AK007836 ncRNA in relation to Pcna.
- To determine if the regulatory function of AK007836 is altered in cancer cells.
Main Methods:
- Analysis of AK007836 and Pcna expression in vitro and in vivo.
- Characterization of the bidirectional promoter activity between AK007836 and Pcna.
- ব্যবহার of small interfering RNA (siRNA) to reduce ncRNA expression and observe effects on Pcna levels.
Main Results:
- AK007836 and Pcna expression showed a positive correlation in both normal and cancer cells.
- A 177 bp intergenic region exhibited bidirectional promoter activity.
- siRNA-mediated knockdown of AK007836 reduced Pcna expression in normal cells but not in cancer cells.
Conclusions:
- AK007836 ncRNA is divergently transcribed from a bidirectional promoter and positively regulates neighboring Pcna gene transcription.
- The regulatory mechanism involving AK007836 and Pcna is impaired in cancer cells.
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