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Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests
Published on: September 28, 2021
Nocardioides lianchengensis sp. nov., an actinomycete isolated from soil
Jian Zhang1, Yuchao Ma2,1, Huimin Yu2
1College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, PR China.
Abstract:
A novel Gram-stain-positive, aerobic, rod-shaped bacterium, designated strain D94-1(T), was isolated from soil collected in Liancheng county, Fujian province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain D94-1(T) was a member of the genus Nocardioides and was most closely related to Nocardioides salarius DSM 18239(T) (98.54% 16S rRNA gene sequence similarity), Nocardioides marinisabuli DSM 18965(T) (98.30%), Nocardioides basaltis KCTC 19365(T) (98.10%) and Nocardioides dokdonensis KCTC 19309(T) (97.76%). Phenotypic characteristics and DNA-DNA relatedness data served to distinguish strain D94-1(T) from N. salarius, N. marinisabuli, N. basaltis and N. dokdonensis. The chemotaxonomic properties of strain D94-1(T) were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid, the predominant menaquinone was MK-8(H(4)) and the major fatty acids were iso-C(16:0) (29.15%) and anteiso-C(17:0) (21.0%). The DNA G+C content was 71.8 mol%. On the basis of the evidence presented in this study, strain D94-1(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides lianchengensis sp. nov. is proposed. The type strain is D94-1(T) (=DSM 24663(T)=CGMCC 4.6858(T)).
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