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Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
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Related Experiment Video

Updated: May 26, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Simultaneous on-chip DC dielectrophoretic cell separation and quantitative separation performance characterization.

Jiashu Sun¹, Yandong Gao, Richard J Isaacs

¹Department of Mechanical Engineering, Vanderbilt University, Nashville, Tennessee 37235-1592, USA.

Analytical Chemistry

|January 11, 2012

Summary

This summary is machine-generated.

This study integrates a microfluidic Coulter counter and dielectrophoretic cell sorter for simultaneous cell separation and sizing. Performance analysis shows that cell sorting accuracy decreases with higher throughput, offering insights for microfluidic device design.

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Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Microfluidic devices are crucial for cell analysis.
Accurate cell separation and sizing are essential for various biological applications.
Existing methods face challenges in throughput and precision.

Purpose of the Study:

To develop and validate an integrated microfluidic system for simultaneous cell separation and sizing.
To characterize the performance of dc-dielectrophoresis (dc-DEP) based cell sorting.
To investigate the impact of sorting throughput on separation efficiency.

Main Methods:

Integration of a MOSFET-based microfluidic Coulter counter with a dc-dielectrophoretic cell sorter.
Testing with polystyrene beads, yeast cells, and a mixture of tumor and bone marrow cells.
Utilizing receiver operator characteristic (ROC) analysis to evaluate separation performance.

Main Results:

Successful simultaneous on-chip cell separation and sizing were demonstrated across diverse samples.
ROC analysis proved effective for characterizing separation of cells with continuous size distribution.
dc-DEP separation performance was found to degrade as sorting throughput increased.

Conclusions:

The integrated system offers a promising platform for high-throughput cell analysis.
Sorting throughput is a critical parameter affecting the accuracy of dc-DEP based cell separation.
Findings provide valuable insights for optimizing microfluidic cell sorter design and operation.