Related Experiment Video

Updated: May 26, 2026

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability
09:49

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

Published on: April 2, 2015

Allowance for effects of thermodynamic nonideality in sedimentation equilibrium distributions reflecting protein

Peter R Wills, David J Scott, Donald J Winzor

    Analytical Biochemistry
    |January 11, 2012
    PubMed

    More Related Videos

    Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry13:26

    Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

    Published on: September 13, 2014

    Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates06:48

    Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates

    Published on: January 5, 2024

    Related Concept Videos

    Extraction: Partition and Distribution Coefficients01:14

    Extraction: Partition and Distribution Coefficients

    The distribution law or Nernst's distribution law is the law that governs the distribution of a solute between two immiscible solvents. This law, also known as the partition law, states that if a solute is added to the mixture of two immiscible solvents at a constant temperature, the solute is distributed between the two solvents in such a way that the ratio of solute concentrations in the solvents remains constant at equilibrium.
    For extracting a solute from an aqueous phase into an organic...
    Chemical Equilibria: Redefining Equilibrium Constant01:20

    Chemical Equilibria: Redefining Equilibrium Constant

    The effect of an inert salt on the solubility of a sparingly soluble salt is known as the salt effect. The degree of the salt effect varies with the ionic strength of the solution, which in turn depends on the activity of the species in the solution. The activity is expressed as the product of concentration and the activity coefficient of the species.
    To calculate the equilibrium constants of solutions of moderately high ionic strength, one must account for the salt effect. This redefined...
    The Equilibrium Binding Constant and Binding Strength02:18

    The Equilibrium Binding Constant and Binding Strength

    The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
    Thermodynamics: Activity Coefficient01:24

    Thermodynamics: Activity Coefficient

    Activity is the measure of the effective concentration of the species in solution. It can be expressed as the product of the molar concentration of the species and its activity coefficient. The activity coefficient is a dimensionless quantity and depends on the total ionic strength of the solution.
    The activity coefficient is a measure of the deviation from ideal behavior. When the ionic strength of the solution is minimal, the activity coefficient of an ionic species is close to unity, making...
    Noncovalent Attractions in Biomolecules02:35

    Noncovalent Attractions in Biomolecules

    Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
    Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
    Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
    Entropy and Solvation02:05

    Entropy and Solvation

    The process of surrounding a solute with solvent is called solvation. It involves evenly distributing the solute within the solvent. The rule of thumb for determining a solvent for a given compound is that like dissolves like. A good solvent has molecular characteristics similar to those of the compound to be dissolved. For example, polar solutions dissolve polar solutes, and apolar solvents dissolve apolar solutes. A polar solvent is a solvent that has a high dielectric constant (ϵ ≥ 15); an...