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Updated: May 26, 2026

Isolation of Mouse Megakaryocyte Progenitors
Published on: May 20, 2021
[mTOR/p70s6k signaling pathway is involved in megakaryocyte polyploidization]
Hui-ying Yu1, Dong-chu Ma, Di Lin
1Department of Experimental Medicine, Northern Hospital, Shen-yang 110840, China. hyingy@sina.com
Aim:
To investigate the mechanisms of megakaryocyte polyploid cell cycle control.
Methods:
The expression and phosphorylation of mTOR/p70s6k pathway proteins was detected by western blot. Double-labeling techniques were used to investigate in which of the phase of the polyploid cell cycle S6K1 at Thr421/Ser424 are phosphorylated.
Results:
Nocodazole induced a relatively synchronized polyploidization in Dami cells. The expression of mTOR and the phosphorylation of mTOR at Ser2448 increased when Dami cells begin to progress from G1 to S-phase in cell cycle. Analysis of flow cytometry showed that phosphorylation of S6K1 at Thr421/Ser424 increased at G2/M-phase.
Conclusion:
mTOR/S6K1 pathway is involved in megakaryocyte polyploid cell cycle control.
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