Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Carriage of multidrug-resistant Gram-negative bacilli: duration and risk factors.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2023
Same author

Analysis of alternariol and alternariol monomethyl ether in foodstuffs by molecularly imprinted solid-phase extraction and ultra-high-performance liquid chromatography tandem mass spectrometry.

Food chemistry·2017
Same author

An automated optofluidic biosensor platform combining interferometric sensors and injection moulded microfluidics.

Lab on a chip·2017
Same author

Comparison of sample preparation strategies for target analysis of total thyroid hormones levels in serum by liquid chromatography-quadrupole time-of-flight-mass spectrometry.

Talanta·2017
Same author

Multiresidue analysis of cephalosporin antibiotics in bovine milk based on molecularly imprinted polymer extraction followed by liquid chromatography-tandem mass spectrometry.

Journal of chromatography. A·2016
Same author

Multiresidue determination of fluoroquinolone antimicrobials in baby foods by liquid chromatography.

Food chemistry·2014
Same journal

Integration of electrochemical sensors in organ-on-a-chip microfluidic platforms: Advances and perspectives.

Biosensors & bioelectronics·2026
Same journal

DNN-PURE: A deep neural network approach to paper-based urea sensing.

Biosensors & bioelectronics·2026
Same journal

Rationally architected MOF-derived Co<sub>3</sub>O<sub>4</sub>@NiMn-LDH hollow heterostructure-based sensor array empowering sensitive detection and discrimination of neurological biomarkers.

Biosensors & bioelectronics·2026
Same journal

Four-in-one multifunctional CoCu-NC@AuPt nanozyme integrated M13 phage-displayed nanobody based multimodal lateral flow immunoassay for bovine lactoferrin detection.

Biosensors & bioelectronics·2026
Same journal

A novel capillary-driven dual-mode imaging flow cytometry system for malaria parasite detection and quantification.

Biosensors & bioelectronics·2026
Same journal

Liver-targeted alkaline phosphatase-activatable fluorescent probe for imaging liver fibrosis and screening anti-fibrotic natural products.

Biosensors & bioelectronics·2026
See all related articles

Related Experiment Video

Updated: May 26, 2026

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip
10:57

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip

Published on: February 7, 2017

Automated portable array biosensor for multisample microcystin analysis in freshwater samples.

S Herranz1, M D Marazuela, M C Moreno-Bondi

  • 1Chemical Optosensors & Applied Photochemistry Group (GSOLFA), Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense de Madrid, E-28040 Madrid, Spain.

Biosensors & Bioelectronics
|January 11, 2012
PubMed
Summary
This summary is machine-generated.

A new automated array biosensor detects microcystins (MCs) in freshwater with high sensitivity and rapid analysis. This environmental monitoring tool offers reliable results comparable to LC-MS/MS.

More Related Videos

Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis
07:18

Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis

Published on: May 31, 2019

Early Detection of Cyanobacterial Blooms and Associated Cyanotoxins using Fast Detection Strategy
07:13

Early Detection of Cyanobacterial Blooms and Associated Cyanotoxins using Fast Detection Strategy

Published on: February 25, 2021

Related Experiment Videos

Last Updated: May 26, 2026

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip
10:57

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip

Published on: February 7, 2017

Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis
07:18

Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis

Published on: May 31, 2019

Early Detection of Cyanobacterial Blooms and Associated Cyanotoxins using Fast Detection Strategy
07:13

Early Detection of Cyanobacterial Blooms and Associated Cyanotoxins using Fast Detection Strategy

Published on: February 25, 2021

Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Microcystins (MCs) are potent cyanotoxins contaminating freshwater sources.
  • Accurate and rapid detection methods are crucial for environmental monitoring and public health protection.

Purpose of the Study:

  • To develop and optimize an automated array biosensor for sensitive and efficient detection of microcystins (MCs) in freshwater samples.
  • To evaluate the biosensor's performance, including sensitivity, specificity, regeneration, and analysis time.

Main Methods:

  • Development of an evanescent-wave excitation-based array biosensor.
  • Covalent immobilization of microcystin-leucine-arginine (MCLR) onto a planar waveguide.
  • Competitive inhibition assay using anti-MCLR monoclonal antibodies and Cy5-labeled detection antibody.
  • Optimization of surface chemistry to enhance performance and minimize non-specific binding.
  • Evaluation of cross-reactivity with other related microcystins.

Main Results:

  • The optimized biosensor achieved an IC(50) of 0.34 ± 0.01 μg/L, a detection limit of 16 ± 3 ng/L, and a dynamic range of 0.06–1.5 μg/L for MCLR.
  • High cross-reactivity was observed for other microcystins (e.g., MCRR at 90%, dm-MCRR at 95%, MCYR at 91%).
  • The automated system processed up to six samples in parallel within approximately 60 minutes.
  • The sensing surface demonstrated excellent reusability, withstanding at least 15 assay-regeneration cycles without significant loss of binding capacity.
  • Successful application to direct analysis of MCs in surface water samples, with results closely matching LC-MS/MS data.

Conclusions:

  • The developed automated array biosensor provides a sensitive, rapid, and reusable platform for microcystin detection in environmental samples.
  • The biosensor's performance, including sensitivity and cross-reactivity profile, makes it a viable alternative to conventional methods like LC-MS/MS for routine monitoring.
  • This technology holds significant potential for safeguarding freshwater quality and protecting public health from microcystin contamination.