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Updated: May 26, 2026

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Application of fast liquid chromatography for antioxidants analysis
Agnieszka Drożdżyńska1, Krzysztof Dzidzic, Alicja Kośmider
1Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Poland. agadro@up.poznan.pl
Background:
An intensive development of the Fast Liquid Chromatography (FLC) has been recently observed. It makes possible to reduce time analysis and improve resolution as well as sensitivity. The aim of this study was to separate the chosen antioxidants optimization using the FLC method.
Material And Methods:
The three various procedures for antioxidants analysis were compared. Mobile phases containing aqueous solution of formic acid, acetic acid, acetonitrile, and methanol were tested. Limit of detection (LOD), limit of quantification (LOQ), linearity and repeatability of each procedures were determined.
Results:
Developed procedure enabled to separate all analytes and allowed to get low LOD levels and good repeatability. This procedure was used for antioxidants analysis in buckwheat and buckwheat products.
Conclusion:
Fast Liquid Chromatography allows to reduce time analysis and obtain good validation parameters.
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