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Related Experiment Videos

New developments in affinity chromatography.

C R Lowe1, S J Burton, N Burton

  • 1Institute of Biotechnology, University of Cambridge, UK.

Journal of Molecular Recognition : JMR
|June 1, 1990
PubMed
Summary
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New dye affinity adsorbents were designed using computer-aided molecular design for selective pharmaceutical protein purification. These stable, high-capacity materials offer low leakage for industrial affinity chromatography applications.

Area of Science:

  • Biotechnology
  • Materials Science
  • Chemical Engineering

Background:

  • Affinity chromatography is crucial for pharmaceutical protein purification.
  • Development of stable and selective adsorbents is essential for industrial processes.
  • Existing adsorbents may face limitations in stability and selectivity.

Purpose of the Study:

  • To design and synthesize novel immobilized dye affinity adsorbents.
  • To achieve predictable selectivity for target pharmaceutical proteins.
  • To develop high-capacity, low-leakage adsorbents for industrial-scale purification.

Main Methods:

  • Computer-aided molecular design (CAMD) for ligand design.
  • Synthesis of dye ligands and coupling to perfluoropolymer supports.

Related Experiment Videos

  • Chromatographic operation and performance evaluation of the adsorbents.

    • Main Results:

    • Novel dye ligands with predictable selectivity were designed.
    • Stable perfluoropolymer supports yielded high-capacity adsorbents.
    • Adsorbents demonstrated low leakage, suitable for affinity chromatography.

    Conclusions:

    • The new adsorbents are stable and selective for pharmaceutical protein purification.
    • These materials are anticipated to withstand industrial sanitization and cleaning conditions.
    • The developed adsorbents represent a promising advancement in bioprocess purification technology.