Related Experiment Video
Updated: May 26, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
[Comparing the primer specificity for betaproteobacterial ammonia-oxidizing bacteria in recirculation aquaculture
Fangfang Pei1, Peng Zhu, Hang Min
1Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China. peifangfang1234@163.com
Objective:
To analyze the difference of specificity of four primers for betaproteobacterial ammonia-oxidizing bacteria (beta-AOB) 16S rDNA gene from the biofilm of closed recirculation aquaculture systems.
Methods:
We used 16S rDNA clone libraries to describe the beta-AOB diversity.
Results:
CTO189f/CTO654r produced the highest frequency of beta-AOB-like sequences (67.3%). The amplification performance of primer was noticeably influenced by the biofilm samples. Hereinto, the biofilm of closed recirculation aquaculture systems of Tilapia nilotica resulted in the higher amplification performance of primers.
Conclusion:
CTO189f/CTO654r exhibited the highest specific for beta-AOB 16S rDNA gene from the biofilm of closed recirculation aquaculture systems.
Related Concept Videos
Metabolism of Chemolithotrophs
Bioreactor Design and Operational System
Anoxygenic Phototrophic Bacteria

