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Related Experiment Video

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A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

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Evaluation of non cyanide methods for hemoglobin estimation.

Vinaya B Shah¹, Bipin S Shah, G V Puranik

¹Department of Pathology, T N Medical College & B Y L Nair Hospital, Mumbai, India. shahvinaya@yahoo.com

Indian Journal of Pathology & Microbiology

|January 12, 2012

Summary

New non-cyanide hemoglobin (Hb) estimation methods offer a safe and reliable alternative to the traditional hemoglobincyanide (HiCN) method. These cyanide-free approaches are cost-effective and suitable for routine laboratory use and point-of-care testing.

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Area of Science:

Clinical Chemistry
Hematology
Analytical Chemistry

Background:

The hemoglobincyanide (HiCN) method is a widely used standard for hemoglobin estimation.
However, the disposal of cyanide-containing reagents poses a significant toxic hazard.
There is a need for safer, non-cyanide alternatives for hemoglobin measurement.

Purpose of the Study:

To evaluate non-cyanide methods for hemoglobin estimation as alternatives to the HiCN method.
To assess the feasibility of alkaline hematin detergent (AHD-575) and alkaline borax methods.
To compare these novel methods with the established cyanmethemoglobin method.

Main Methods:

Two cyanide-free hemoglobin estimation methods were developed and tested.
Hemoglobin levels in 200 samples were compared using these methods and the cyanmethemoglobin method.
Additional comparisons were made on blood donors and infant blood samples.
Statistical analysis involved Pearson's correlation coefficient.

Main Results:

Non-cyanide methods demonstrated linear response across a wide hemoglobin concentration range (3-20 g/dL).
These methods showed high precision (+0.25 g/dL, CV=2.34%) and excellent correlation (r=0.98) with the HiCN method.
Reagents are non-biohazardous, cost-effective, and suitable for colorimeters at 530 nm and 580 nm.

Conclusions:

Non-cyanide hemoglobin estimation methods provide a safe and reliable alternative for routine laboratory use.
These methods are suitable for point-of-care testing, particularly in blood banks for blood donors.
The adoption of these methods can mitigate the toxic hazards associated with cyanide reagents.