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Related Experiment Video

Updated: May 25, 2026

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
07:06

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Published on: April 3, 2017

Mesenchymal stem cell isolation and characterization from human spinal ligaments.

Toru Asari1, Ken-Ichi Furukawa, Sunao Tanaka

  • 1Department of Orthopaedic Surgery, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan.

Biochemical and Biophysical Research Communications
|January 12, 2012
PubMed
Summary

Researchers isolated mesenchymal stem cells (MSCs) from human spinal ligaments, demonstrating their potential for differentiation and identifying their location within the ligament tissue. This discovery aids in understanding spinal diseases.

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Area of Science:

  • Orthopedics and Regenerative Medicine
  • Stem Cell Biology

Background:

  • Mesenchymal stem cells (MSCs) are known for their self-renewal and differentiation capabilities.
  • Previous research has documented MSC isolation from various human tissues, but not spinal ligaments.
  • Spinal ligament ossification and hypertrophy are poorly understood conditions.

Purpose of the Study:

  • To isolate and characterize mesenchymal stem cells (MSCs) from human spinal ligaments.
  • To determine the in situ localization of MSCs within spinal ligament tissue.
  • To explore the potential role of these cells in spinal pathologies.

Main Methods:

  • Human spinal ligaments were aseptically harvested from patients undergoing surgery.
  • Cells were isolated via collagenase digestion and cultured to assess expansion and senescence.
  • Multilineage differentiation potential (osteogenic, adipogenic, chondrogenic) was evaluated.
  • Immunophenotypic analysis using flow cytometry and immunohistochemistry (CD90) was performed.

Main Results:

  • Mesenchymal stem cells (MSCs) were successfully isolated and extensively expanded from human spinal ligaments with limited senescence.
  • Isolated cells demonstrated multilineage differentiation potential into osteogenic, adipogenic, and chondrogenic lineages.
  • Flow cytometry confirmed that the cells met the minimum criteria for MSCs.
  • Immunohistochemistry identified CD90-positive cells within the ligament's collagenous matrix and adjacent blood vessels.

Conclusions:

  • Mesenchymal stem cells (MSCs) can be isolated and expanded from human spinal ligaments.
  • These spinal ligament-derived MSCs possess characteristic stem cell properties, including differentiation potential and specific surface markers.
  • The localization of MSCs in spinal ligaments suggests their involvement in ligament homeostasis and potentially in the pathogenesis of spinal diseases like ossification.