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Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+

Monitoring ER/SR Calcium Release with the Targeted Ca²⁺ Sensor CatchER⁺

Published on: May 19, 2017

Colorful calcium sensors.

Laurens Lindenburg¹, Maarten Merkx

¹Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, Den Dolech 2, 5612 AZ Eindhoven (The Netherlands).

Chembiochem : a European Journal of Chemical Biology

|January 12, 2012

Summary

Researchers developed new genetically encoded calcium sensors using directed evolution. This expands the available tools for studying calcium signaling, including the first red-shifted sensor.

Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

Calcium signaling is crucial for various cellular processes.
Genetically encoded calcium sensors (GECSs) are vital tools for studying these signals.
Expanding the diversity of GECSs, particularly with new colors, is essential for advanced research.

Purpose of the Study:

To develop novel genetically encoded calcium sensors.
To create a broader palette of sensors with improved or new characteristics.
To introduce the first red-shifted GECSs for expanded research capabilities.

Main Methods:

Utilized a novel directed-evolution strategy.
Applied selection and screening techniques to identify improved sensor variants.

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Focused on developing sensors with distinct spectral properties, including red-shift.

Main Results:

Successfully generated a diverse collection of new genetically encoded calcium sensors.
Developed the first red-shifted genetically encoded calcium sensor.
Demonstrated the utility of these new sensors for calcium signaling research.

Conclusions:

The new directed-evolution approach effectively expanded the toolbox of GECSs.
The availability of new colors, especially red-shifted variants, enhances the study of complex calcium dynamics.
These advancements will facilitate deeper understanding of calcium signaling and its interplay with other cellular pathways.