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Related Experiment Video

Updated: May 25, 2026

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

Towards a "Sample-In, Answer-Out" Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV

Anja Gulliksen¹, Helen Keegan, Cara Martin

¹NorChip AS, Industriveien 8, 3490 Klokkarstua, Norway.

Journal of Oncology

|January 12, 2012

Summary

This study developed an automated, hands-free diagnostic platform for detecting human papillomavirus (HPV) mRNA in clinical samples. The system successfully performed sample preparation, amplification, and detection, demonstrating a "sample-in, answer-out" capability.

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Last Updated: May 25, 2026

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Published on: October 8, 2015

Area of Science:

Biotechnology
Molecular Diagnostics
Medical Devices

Background:

Cervical cancer screening relies on detecting high-risk human papillomavirus (HPV) types.
Current diagnostic methods often require complex laboratory procedures and skilled personnel.
There is a need for automated, user-friendly platforms for efficient HPV detection.

Purpose of the Study:

To develop and demonstrate a proof-of-principle, hands-free, and self-contained diagnostic platform for HPV E6/E7 mRNA detection.
To integrate automated sample preconcentration, nucleic acid extraction, amplification, and real-time detection.
To validate the platform's performance using clinical cervical cytology specimens.

Main Methods:

Development of two modular prototypes: one for sample preparation and one for amplification and detection.
Chip-based automation of sample preconcentration and nucleic acid extraction.
Real-time fluorescent detection using NASBA (Nucleic Acid Sequence-Based Amplification) technology.

Main Results:

The sample preparation module achieved HPV detection rates of 50%-85.7% across dilutions in 28 cervical cytology specimens.
Complete validation on the NASBA chip module showed 100% amplification for HPV 16 or 33 in 4 out of 6 clinical samples at a 1:10 dilution.
Successful demonstration of the integrated workflow from clinical sample input to final detection.

Conclusions:

The developed modular platform demonstrates the feasibility of a "sample-in, answer-out" diagnostic system for HPV mRNA detection.
The hands-free and automated nature of the platform minimizes user intervention, enhancing potential for widespread clinical use.
Further integration and optimization of the modules can lead to a fully automated, point-of-care HPV diagnostic solution.