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High phosphorus diet-induced changes in NaPi-IIb phosphate transporter expression in the rat kidney: DNA microarray
Tatsuya Suyama1, Shinji Okada, Tomoko Ishijima
1Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Plos One
|January 12, 2012
Summary
High phosphorus diets increase phosphorus excretion in rats, not retention. Kidney gene expression analysis reveals upregulation of the sodium-dependent phosphate transporter NaPi-IIb, suggesting its role in active phosphate excretion.
Area of Science:
- Nephrology
- Molecular Biology
- Physiology
Background:
- Maintaining phosphorus homeostasis is crucial for overall health.
- Dietary phosphorus intake significantly impacts bodily phosphorus levels.
- The kidney plays a key role in regulating phosphorus excretion.
Purpose of the Study:
- To investigate the kidney's response to a high phosphorus (HP) diet at the gene expression level.
- To identify specific mechanisms involved in phosphorus regulation under HP conditions.
- To elucidate the role of the sodium-dependent phosphate transporter (NaPi-IIb) in HP-induced phosphorus excretion.
Main Methods:
- Male Wistar rats were fed either a control or a high phosphorus diet for 24 days.
- Fractional phosphorus excretion was measured.
- DNA microarray analysis was performed on kidney tissue to assess gene expression changes.
- In situ hybridization and immunohistochemistry were used to confirm NaPi-IIb expression and localization.
Main Results:
- HP diet led to increased fractional phosphorus excretion, indicating active excretion rather than retention.
- Microarray analysis revealed significant changes in kidney gene expression profiles.
- Genes related to ossification, collagen organization, and immune response were upregulated.
- Type IIb sodium-dependent phosphate transporter (NaPi-IIb) was significantly upregulated in the kidneys of HP-fed rats.
- NaPi-IIb was localized to the basolateral side of kidney epithelial cells in HP rats.
Conclusions:
- The kidney actively excretes excess phosphorus when exposed to a high phosphorus diet.
- Upregulation of NaPi-IIb in the kidney is a key adaptive response to high dietary phosphorus.
- NaPi-IIb plays a significant role in the renal excretion of phosphate.
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