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Related Experiment Video

Updated: May 25, 2026

The Immediate Partial Removal of Cumulus-Oocyte Complexes: A Refined Approach for Rapid Observation of In Vitro Fertilization

The Immediate Partial Removal of Cumulus-Oocyte Complexes: A Refined Approach for Rapid Observation of In Vitro Fertilization

Published on: October 18, 2024

[Failed fertilization after ICSI: causes and countermeasures].

Dan Cheng¹, Jie Li, Cui-Cui Guo

¹Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. chengdanny@126.com

Zhonghua Nan Ke Xue = National Journal of Andrology

|January 13, 2012

Summary

Intracytoplasmic sperm injection (ICSI) offers high monospermy rates in IVF but doesn't eliminate fertilization failures. Causes include poor gamete quality, activation issues, or ICSI technique problems, presenting ongoing challenges.

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Area of Science:

Reproductive biology
Assisted reproductive technology

Context:

Intracytoplasmic sperm injection (ICSI) is a key technique in in vitro fertilization (IVF).
While ICSI improves monospermy rates, fertilization failures can still occur.
Factors contributing to failure include oocyte/sperm quality, activation issues, and technical challenges.

Purpose:

To explore the causes of fertilization failure following ICSI.
To discuss methods for overcoming oocyte activation failure.
To address sperm-related fertilization issues in ICSI.

Summary:

ICSI, a refined IVF method, achieves high monospermy but cannot fully prevent fertilization failures.
Potential causes include poor oocyte or sperm quality, abnormal oocyte activation, and ICSI technical difficulties.
Controlled ovarian hyperstimulation may increase oocyte apoptosis, leading to fertilization failure, while morphologically selective ICSI addresses sperm defects.

Impact:

Highlights the persistent challenge of total fertilization failure post-ICSI.
Suggests the need for further research into preventing and managing ICSI-related fertilization failures.
Informs clinical practice regarding potential causes and solutions for ICSI outcomes.