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Updated: Aug 18, 2026

10:51
Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
Published on: November 15, 2013
[Interaction between the dexamethasone-receptor complex and Zajdela ascites hepatoma DNA]
Biokhimiia (Moscow, Russia)
|May 1, 1979
Abstract:
The activated dexamethasone--receptor complex from rat liver cell cytoplasm and from Zajdela ascite hepatoma cell cytoplasm is bound to Sepharose-immobilized native DNA containing different numbers of recurrent sequences. The DNA-bound hormonereceptor complex is eluted as three peaks by varying concentrations of NaCl. The binding in independent of the number of DNA recurrent sequences and of the source of the cytoplasmic complex used.

