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Infectivity of DNA recovered from cells persistently infected with SV5 paramyxovirus
Abstract:
Infectivity of DNA isolated from L cells chronically infected with SV5 paramyxovirus was demonstrated by inoculation of continuous RH and HEp-2 cells. Infectivity of the DNA was completely abolished by treatment with deoxyribonuclease or by alkaline hydrolysis but did not change after treatment with ribonuclease and specific anti SV5 serum. The virus obtained as a result of transfection caused haemadsorption in susceptible cells and was neutralized by specific antiserum like the prototype SV5 strain.
Insights
Infectious DNA from simian virus 5 (SV5) paramyxovirus was isolated and shown to infect cells. DNA infectivity was confirmed by its susceptibility to deoxyribonuclease and alkaline hydrolysis, proving it carries the viral genetic material.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Simian virus 5 (SV5) is a paramyxovirus.
- Chronic infection models are crucial for studying persistent viral agents.
- Understanding viral DNA infectivity is key to viral replication mechanisms.
Purpose of the Study:
- To demonstrate the infectivity of DNA isolated from SV5-infected cells.
- To characterize the nature of the infectious agent within the isolated DNA.
- To confirm the genetic material responsible for SV5 propagation.
Main Methods:
- Isolation of DNA from SV5-chronically infected L cells.
- Transfection of continuous RH and HEp-2 cell lines with isolated DNA.
- Enzymatic treatments (deoxyribonuclease, ribonuclease) and alkaline hydrolysis of DNA.
- Neutralization assays with anti-SV5 antiserum.
- Assessment of viral properties like haemadsorption.
Main Results:
- Infectivity of isolated DNA was demonstrated in recipient cells.
- DNA infectivity was abolished by deoxyribonuclease and alkaline hydrolysis, indicating DNA as the infectious agent.
- Ribonuclease and anti-SV5 serum treatments did not affect DNA infectivity.
- Transfected virus exhibited characteristic SV5 properties, including haemadsorption and neutralization by specific antiserum.
Conclusions:
- The genetic material of SV5 paramyxovirus is DNA.
- Isolated DNA from chronically infected cells is infectious and capable of producing infectious virions.
- This study confirms DNA as the carrier of genetic information for SV5 replication.