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Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Related Experiment Video

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Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside
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A phenol-chloroform protocol for extracting DNA from ancient samples.

Ross Barnett1, Greger Larson

  • 1Department of Archaeology, Durham University, South Road, Durham, DH1 3LE, UK. drrossbarnett@gmail.com

Methods in Molecular Biology (Clifton, N.J.)
|January 13, 2012
PubMed
Summary
This summary is machine-generated.

Ancient DNA (aDNA) extraction is crucial for sequencing. The phenol-chloroform method effectively isolates aDNA from diverse tissues, preserving valuable low molecular weight DNA for downstream applications.

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Area of Science:

  • Paleogenomics
  • Molecular Biology
  • Biochemistry

Background:

  • Ancient DNA (aDNA) analysis is vital for numerous scientific applications.
  • DNA extraction from ancient materials like bone, teeth, and hair is a foundational step.
  • Existing protocols show variable success based on tissue type and preservation conditions.

Purpose of the Study:

  • To present a robust DNA extraction method applicable to all ancient tissue types.
  • To detail the phenol-chloroform technique for ancient DNA recovery.

Main Methods:

  • Utilized the phenol-chloroform extraction protocol.
  • Applied the method to various ancient tissue types.
  • Focused on recovery of total nucleic acid content.

Main Results:

  • The phenol-chloroform method demonstrated effectiveness across different ancient tissues.
  • This technique allows for the recovery of total nucleic acid.
  • Minimal loss of low molecular weight double-stranded DNA was observed.

Conclusions:

  • The phenol-chloroform method is a versatile and reliable technique for ancient DNA extraction.
  • This protocol supports diverse downstream applications requiring high-quality aDNA.
  • It offers a consistent approach for DNA recovery from challenging ancient samples.