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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Related Experiment Video

Updated: May 25, 2026

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

Published on: March 26, 2011

DNA extraction from keratin and chitin.

Paula F Campos¹, Thomas M P Gilbert

¹Natural History Museum of Denmark, University of Copenhagen, Østervoldgade 5-7, 1350 Copenhagen, Denmark.

Methods in Molecular Biology (Clifton, N.J.)

|January 13, 2012

Summary

Extracting DNA from keratinous and chitinous sources is possible using specialized buffers. These buffers contain DTT, proteinase K, and detergent, followed by silica-column or organic purification for genetic analysis.

Area of Science:

Molecular Biology
Forensic Science
Genetics

Background:

Keratinous and chitinous materials are often sources of valuable genetic information.
Efficient DNA extraction from these complex matrices remains a challenge.

Purpose of the Study:

To outline effective methods for liberating and purifying DNA from keratinous and chitinous samples.
To provide recommendations for optimizing DNA yield and quality from challenging biological materials.

Main Methods:

Utilizing extraction buffers with high concentrations of DTT (dithiothreitol), proteinase K, and detergent.
Employing purification techniques such as silica-column chromatography or organic extraction methods.

Main Results:

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Last Updated: May 25, 2026

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High levels of DTT, proteinase K, and detergent effectively liberate DNA from keratinous and chitinous materials.

Both silica-column and organic purification methods yield successful DNA recovery.

Conclusions:

Recommended buffer compositions and purification strategies enable reliable DNA extraction from keratinous and chitinous sources.
This facilitates the broader use of these materials for genetic information retrieval in various scientific disciplines.