Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Early colonization before inundation consistent with northern glacial refugia in Southern Doggerland revealed by sedimentary ancient DNA.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

A legacy of genetic entanglement with wolves shapes modern dogs.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

Population structure and inter-species admixture within a likely extinct yet formerly widespread Hawaiian honeycreeper.

Biology letters·2025

Same author

Museum Genomics Reveals Temporal Genetic Stasis and Global Genetic Diversity in Arabidopsis thaliana.

Molecular ecology·2025

Same author

Historic manioc genomes illuminate maintenance of diversity under long-lived clonal cultivation.

Science (New York, N.Y.)·2025

Same author

Early evidence of avocado domestication from El Gigante Rockshelter, Honduras.

Proceedings of the National Academy of Sciences of the United States of America·2025

Search research articles

Related Experiment Video

Updated: Nov 12, 2025

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

Published on: November 1, 2024

Ancient DNA extraction from plants.

Logan Kistler¹

¹Department of Anthropology, The Pennsylvania State University, 409 Carpenter Building, University Park, PA 16802, USA. ljk187@psu.edu

Methods in Molecular Biology (Clifton, N.J.)

|January 13, 2012

Summary

This summary is machine-generated.

Two DNA extraction protocols are presented for ancient plant specimens. The CTAB method works well for single seeds, while the PTB method is effective for gourd rind and similar tissues, optimizing ancient DNA recovery.

More Related Videos

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

Related Experiment Videos

Last Updated: Nov 12, 2025

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

Published on: November 1, 2024

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

Area of Science:

Paleogenomics
Archaeobotany
Molecular Biology

Background:

Ancient plant DNA extraction presents challenges due to diverse preservation conditions and tissue types.
Standard commercial DNA extraction kits may require modifications for optimal ancient DNA yield.

Purpose of the Study:

To describe two effective DNA extraction protocols for small amounts of ancient plant tissue.
To provide optimized methods for recovering ancient DNA from archaeobotanical samples.

Main Methods:

CTAB protocol: Incubation in extraction buffer, chloroform extraction, DNA purification, and suspension, suitable for single seeds.
PTB protocol: Overnight incubation of pulverized tissue, centrifugation, and subsequent DNA extraction using commercial kits, recommended for gourd rind.

Main Results:

Both CTAB and PTB protocols demonstrate efficacy in extracting DNA from challenging ancient plant materials.
The described methods aim to improve DNA yield from limited archaeological and paleobotanical specimens.

Conclusions:

The CTAB and PTB protocols offer valuable, tailored approaches for ancient plant DNA extraction.
These methods enhance the potential for genetic analysis of ancient flora from diverse samples.