Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleotide sequence and expression of a cDNA encoding chick brain actin depolymerizing factor.

M E Adams1, L S Minamide, G Duester

  • 1Department of Biochemistry, Colorado State University, Fort Collins 80523.

Biochemistry
|August 14, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rebuttal to: Simvastatin Treatment Does Not Ameliorate Muscle Pathophysiology in a Mouse Model for Duchenne Muscular Dystrophy, Verhaart et al. 2020.

Journal of neuromuscular diseases·2021
Same author

RanBP9 at the intersection between cofilin and Aβ pathologies: rescue of neurodegenerative changes by RanBP9 reduction.

Cell death & disease·2015
Same author

Susceptibility-weighted imaging changes suggesting brain iron accumulation in Huntington's disease: an epiphenomenon which causes diagnostic difficulty.

European journal of neurology·2014
Same author

Role of SNTA1 in Rac1 activation, modulation of ROS generation, and migratory potential of human breast cancer cells.

British journal of cancer·2014
Same author

Chemonucleolysis.

Canadian Medical Association journal·2010
Same author

Autotrophic growth of sulphate-reducing bacteria.

Nature·2010
Same journal

Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

Biochemistry·2026
Same journal

Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

Biochemistry·2026
Same journal

Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

Biochemistry·2026
Same journal

Discovery of Bacterial Unspecific Peroxygenases.

Biochemistry·2026
Same journal

Lactate Biology: Subcellular Routing and Chemical Form Define Function.

Biochemistry·2026
Same journal

Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

Biochemistry·2026
See all related articles

Researchers identified the chick brain actin depolymerizing factor (ADF) gene. This protein severs actin filaments and shows high similarity to cofilin, indicating conserved functions in actin dynamics.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Background:

  • Actin depolymerizing factor (ADF) is crucial for regulating actin dynamics in cells.
  • Understanding ADF's structure and function is key to comprehending cellular processes like motility and shape.
  • Chick ADF shares similarities with porcine cofilin, suggesting conserved roles in actin binding proteins.

Purpose of the Study:

  • To isolate and characterize the cDNA encoding chick brain actin depolymerizing factor (ADF).
  • To analyze the deduced amino acid sequence and compare it with known actin-binding proteins.
  • To confirm the functional activity and expression of the cloned ADF.

Main Methods:

  • Screening a chick embryo cDNA library using specific antibodies and oligonucleotide probes.

Related Experiment Videos

  • DNA sequencing of isolated cDNA clones to determine the full-length sequence.
  • Expression of ADF in a vector, followed by protein purification and functional assays.
  • Northern and Southern blotting to analyze gene expression and copy number.

    • Main Results:

    • A full-length cDNA encoding chick ADF (165 amino acids) was isolated and sequenced.
    • The deduced ADF sequence exhibits 73% identity to porcine brain cofilin.
    • Expressed ADF demonstrated actin depolymerizing activity and immunoreactivity with anti-ADF antiserum.
    • Two ADF mRNA transcripts (2.1 and 0.9 kb) were detected in embryonic chick tissues.

    Conclusions:

    • Chick brain ADF is a distinct protein with conserved functional domains similar to cofilin.
    • The ADF gene is likely a single copy in the chicken genome.
    • ADF plays a significant role in regulating actin dynamics in chick embryonic tissues.