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Updated: May 25, 2026

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
Published on: January 17, 2025
Discovery of an entropically-driven small molecule streptavidin binder from nucleic acid-encoded libraries
Jean-Pierre Daguer1, Mihai Ciobanu, Sofia Barluenga
1Institut de Science et Ingenierie Supramoleculaires (ISIS), Université de Strasbourg-CNRS (UMR 7006), 8 allee Gaspard Monge, 67000 Strasbourg, France.
Abstract:
Dehydrocholic acid was identified as a selective streptavidin binder from a PNA-tagged library. Isothermal calorimetry titration measurements showed this interaction to be entropically driven. Peptides tagged with dehydrocholic acid can be captured on a streptavidin resin and released under thermal conditions.
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