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Related Experiment Video

Updated: May 25, 2026

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

Expanding and engineering the genetic code in a single expression experiment.

Michael G Hoesl¹, Nediljko Budisa

¹Max Planck Institute of Biochemistry, Molecular Biotechnology, Am Klopferspitz 18, 82152 Martinsried, Germany.

Chembiochem : a European Journal of Chemical Biology

|January 13, 2012

Summary

This summary is machine-generated.

Researchers engineered and expanded the genetic code in a single experiment. They achieved specific amino acid substitutions and incorporated unnatural amino acids using amber suppressor tRNA.

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Last Updated: May 25, 2026

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

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Published on: April 27, 2018

Area of Science:

Synthetic biology
Genetic engineering
Molecular biology

Background:

The genetic code dictates protein synthesis.
Modifying the genetic code allows for novel protein functionalities.
Simultaneous code engineering and expansion presents a significant challenge.

Purpose of the Study:

To demonstrate the simultaneous engineering and expansion of the genetic code in a single in vivo experiment.
To combine sense codon reassignment with STOP codon read-through for enhanced protein synthesis.

Main Methods:

Utilized residue-specific sense codon reassignments (Met→Nle and Pro→(4S-F)Pro) for code engineering.
Employed position-specific STOP codon read-through using an amber suppressor tRNA for code expansion.
Performed a single in vivo expression experiment to validate the combined approach.

Main Results:

Successfully achieved simultaneous genetic code expansion and engineering.
Demonstrated specific amino acid substitutions and incorporation of unnatural amino acids.
Validated the feasibility of combining codon reassignment and STOP codon read-through in vivo.

Conclusions:

The study presents a novel method for simultaneously expanding and engineering the genetic code.
This approach opens new avenues for creating proteins with tailored properties.
The findings pave the way for advanced synthetic biology applications.