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Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
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Elevated CD14++CD16- monocytes predict cardiovascular events.

Katarina E Berg1, Irena Ljungcrantz, Linda Andersson

  • 1Department of Clinical Sciences, Skåne University Hospital Malmö, Lund University, Malmö, Sweden.

Circulation. Cardiovascular Genetics
|January 13, 2012
PubMed
Summary
This summary is machine-generated.

Classical CD14(++)CD16(-) monocytes can predict future cardiovascular events. Higher numbers of these monocytes indicate increased cardiovascular risk, independent of other known risk factors in a general population.

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Area of Science:

  • Immunology
  • Cardiovascular Medicine
  • Epidemiology

Background:

  • Peripheral blood monocytes are heterogeneous, but links between specific subsets and cardiovascular disease (CVD) risk are understudied in large populations.
  • Previous research has not sufficiently highlighted the prognostic value of distinct monocyte subsets in epidemiological studies concerning CVD.

Purpose of the Study:

  • To investigate the association between monocyte subsets and the incidence of ischemic cardiovascular events.
  • To determine if specific monocyte populations can serve as independent predictors of future cardiovascular risk.

Main Methods:

  • Analysis of 700 randomly selected subjects from the Malmö Diet and Cancer study.
  • Flow cytometry was used to enumerate monocyte subsets (CD14 and CD16 expression) from cryopreserved leukocytes.
  • Ischemic cardiovascular events were tracked until December 2008.

Main Results:

  • Subjects experiencing cardiovascular events had increased percentages and numbers of classical CD14(++)CD16(-) monocytes compared to event-free individuals.
  • A higher number of CD14(++)CD16(-) monocytes was associated with a 1.66-fold increased hazard of cardiovascular events, even after adjusting for traditional risk factors.
  • Classical CD14(++)CD16(-) monocytes did not correlate with atherosclerosis extent at baseline, but CD16 expression on monocytes showed a negative association with carotid intima-media thickness.

Conclusions:

  • Classical CD14(++)CD16(-) monocytes are significant predictors of future cardiovascular risk.
  • This monocyte subset provides predictive value for cardiovascular events independently of established risk factors in a general population.