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Updated: May 25, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Investigation on the chemiluminescence reaction of the phenylhydrazine-luminol-peroxide system
A L S Chandel1, S A Khan, R S Kher
1Department of Chemistry, Government E. R. R. Science P. G. College, Bilaspur, India.
Abstract:
We studied the chemiluminescence (CL) oxidation of phenyl hydrazine-luminol with various organic and inorganic peroxides. Maximum CL intensity for this system was obtained for t-butylhydroperoxide. The enhancement in CL depended strongly on pH and was greatest at pH 12.5. The solvent drastically enhanced the CL intensity. DMSO was found to increase the CL intensity many-fold as compared to acetonitrile and water. The effect of temperature on CL intensity has also been studied. The CL spectra revealed a broad peak at 425 nm, which suggests excited 3-aminophthalate ion as the luminophor. A mechanism to explain the reactions is suggested.
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