[Heparin-sepharose affinity chromatography and reduction conditions as a method of a selective process for separate...

Gel permeation chromatography of VLDL apoproteins on Sepharose CL-6B in denaturing conditions and affinity chromatography on heparin-sepharose 4B in the presence of reducing agent dithiothreitol were used for preparative isolation of apolipoprotein E of high purity from human plasma VLDL. Sequential elution of apolipoprotein E from affinity column using increasing ionic strength solutions (0.4 M NaCl and 1.0 M NaCl) permitted to obtain "high affinity" apo E preparation with increased relative apoprotein isoform content with the highest positive charge.