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Updated: May 25, 2026

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens
Published on: June 7, 2017
[The efficacy of three hand asepsis techniques using chlorhexidine gluconate (CHG 2%)]
Érika Rossetto da Cunha1, Fabiana Gonçalves de Oliveira Azevedo Matos, Adriana Maria da Silva
1Programa de Pós-Graduação, Escola de Enfermagem, Universidade de São Paulo, São Paulo, SP, Brasil. derika.feres@terra.com.br
Abstract:
The scrubbing of hands and forearms using antiseptic agents has been the standard pre-operative procedure to prevent surgical site infection. With the introduction of antiseptic agents, the need to use brushes for pre-operative disinfection has been questioned and it has been recommended that the procedure be abandoned due to the injuries it may cause to the skin. With the purpose to provide the foundations for the efficacy of pre-operative asepsis without using brushes or sponges, the objective of this study was to evaluate three methods of pre-operative asepsis using an antimicrobial agent containing chlorhexidine gluconate - CHG 2%; hand-scrubbing with brush (HSB), hand-scrubbing with sponge (HSS), and hand-rubbing with the antiseptic agent (HRA) only. A comparative crossover study was carried with 29 healthcare providers. Antimicrobial efficacy was measured using the glove-juice method before and after each tested method. Statistical analyses showed there were no significant differences regarding the number of colony-forming units when comparing HRA, HSB, and HSS techniques (p=0.148), which theoretically disregards the need to continue using brushes or sponges for hand asepsis.
Insights
Pre-operative hand disinfection using antiseptic agents like chlorhexidine gluconate (CHG) can be effective without brushes or sponges. Hand-rubbing with CHG 2% showed comparable antimicrobial efficacy to scrubbing with brushes or sponges, supporting its use in surgical asepsis.
Area of Science:
- Infection Control
- Microbiology
- Surgical Hygiene
Background:
- Pre-operative hand disinfection is crucial for preventing surgical site infections.
- The use of brushes and sponges in pre-operative disinfection has been questioned due to potential skin injury.
- Antiseptic agents, particularly chlorhexidine gluconate (CHG), are widely used.
Purpose of the Study:
- To evaluate the efficacy of pre-operative hand asepsis without brushes or sponges.
- To compare three methods: hand-scrubbing with brush (HSB), hand-scrubbing with sponge (HSS), and hand-rubbing with antiseptic agent (HRA) using 2% CHG.
- To provide evidence for brush-free and sponge-free pre-operative disinfection protocols.
Main Methods:
- A comparative crossover study involving 29 healthcare providers.
- Evaluation of antimicrobial efficacy using the glove-juice method.
- Comparison of colony-forming units before and after three distinct hand asepsis techniques.
Main Results:
- No significant differences in antimicrobial efficacy were observed between hand-rubbing with antiseptic agent (HRA), hand-scrubbing with brush (HSB), and hand-scrubbing with sponge (HSS).
- Statistical analysis (p=0.148) indicated comparable reduction in microbial load across all tested methods.
- The results support the potential to eliminate brushes and sponges in pre-operative hand asepsis.
Conclusions:
- Hand-rubbing with 2% chlorhexidine gluconate (CHG) is as effective as traditional scrubbing methods for pre-operative hand asepsis.
- The findings suggest that brushes and sponges may not be necessary for effective pre-operative disinfection.
- This study supports the adoption of simpler, potentially less injurious, hand asepsis techniques in healthcare settings.
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