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Updated: May 25, 2026
Murine Model of Advanced Periodontitis Induced by Nylon Ligature in the Second Upper Molar
Published on: May 30, 2025
[Experimental study on the functional regulation of naringin in human periodontal ligament cells]
Ang Li1, Jun-jie Zhao, Jin Liu
1Research Center of Stomatology, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, Shanxi Province, China. drliang@mail.xjtu.edu.cn
Purpose:
To detect the effect of naringin on human periodontal ligament cells' (hPDLCs) proliferation,bone formation and OPG mRNA expression.
Methods:
hPDLCs were primarily cultured and identified in vitro through enzyme digestion combined tissue culture method. With 3-(4, 5-Dimethylthiazol-2- yl)-2, 5-diphenyltetrazolium bromide (MTT) method, enzyme linked immunosorbent assay and RT-PCR, the hPDLCs' proliferation, alkaline phosphatase (ALP) activity, expression of collagen protein-I and OPG mRNA were observed after treatment with different concentrations (100,10,1.0,0.1,0.01mg/L) of naringin at different times. SPSS16.0 software package was used for statistical analysis.
Results:
Primary cultured hPDLCs had good shape; Significant promotion of proliferation,ALP activity and collagen protein-I expression of hPDLCs with naringin was found at the dose of 1.0 mg/L; and 1.0mg/L naringin regulated the expression of OPG mRNA in time-dependent manner.
Conclusion:
Naringin might significantly promote the proliferation of hPDLCs and conversion into the osteoblast.
