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Related Experiment Videos

Flow cytometry: its applications to immunohaematology.

G Garratty

    Bailliere'S Clinical Haematology
    |April 1, 1990
    PubMed
    Summary
    This summary is machine-generated.

    Flow cytometry offers a sensitive and objective method for immunohaematology. This technique accurately detects cell-bound antibodies, minor cell populations, and cellular antigens, improving diagnostic capabilities.

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    Area of Science:

    • Immunohaematology
    • Cellular Immunology
    • Analytical Cytology

    Background:

    • Flow cytometry, utilizing fluorescent probes, is a recent advancement in immunohaematology.
    • Its application extends to detecting and quantifying cell-bound immunoglobulins, complement, minor cell populations, cellular antigens, and antibodies against platelets and granulocytes.

    Purpose of the Study:

    • To highlight the practical applications of flow cytometry in immunohaematology.
    • To demonstrate its utility in detecting various cellular components and antibodies.
    • To assess its potential for routine clinical pathology laboratory use.

    Main Methods:

    • Application of flow cytometry with fluorescent probes.
    • Detection and quantitation of cell-bound immunoglobulins and complement.

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  • Identification of minor cell populations, cellular antigens, and antibodies.

    • Main Results:

    • Successful detection of fetal-maternal hemorrhage, red blood cell (RBC) survival, chimerism, and mosaicism.
    • Accurate quantitation of RBC antigens, surpassing traditional serological methods for zygosity determination.
    • Sensitive and reproducible detection of platelet auto- and alloantibodies, granulocyte antibodies, and antibodies to monocytes and lymphocytes.

    Conclusions:

    • Flow cytometry provides a sensitive, objective, and reproducible method for immunohaematological analyses.
    • Its applications in detecting cellular antigens and antibodies offer significant advantages over conventional techniques.
    • Widespread availability of flow cytometers may lead to their routine integration into clinical pathology laboratories.