Ten polymorphic microsatellite loci identified from a small insert genomic library for Peronospora tabacina
Robert N Trigiano1, Phillip A Wadl, Deborah Dean
1Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996-4560, USA. rtrigian@utk.edu
Mycologia
|January 14, 2012
Summary
Ten polymorphic microsatellite loci were identified for Peronospora tabacina, a tobacco pathogen. These markers are suitable for studying genetic diversity and populations of this important oomycete species.
Area of Science:
- * Plant Pathology
- * Oomycete Genetics
- * Molecular Biology
Background:
- * Peronospora tabacina is an obligate biotrophic oomycete pathogen affecting tobacco.
- * Understanding its genetic diversity is crucial for disease management.
Purpose of the Study:
- * To identify and characterize polymorphic microsatellite loci for Peronospora tabacina.
- * To develop molecular markers for population and genetic diversity studies.
Main Methods:
- * Genomic library enrichment for GT microsatellite motifs.
- * Primer design and amplification for identified loci.
- * Characterization of 10 polymorphic loci using 44 P. tabacina isolates from global sources.
Main Results:
- * Identified 162 microsatellite loci, with 85% being dinucleotide repeats.
- * 10 polymorphic loci were characterized, showing 3-4 alleles per locus and heterozygosity ranging from 0.32-0.95.
- * Polymorphic Information Content (PIC) ranged from 0.35-0.69, with a mean of 0.50.
Conclusions:
- * The developed microsatellite markers are effective for genetic diversity and population studies of Peronospora tabacina.
- * These markers may also be applicable to other Peronospora species.
- * The study provides essential tools for oomycete population genetics research.
