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Bacterial contamination of anesthesia machines' internal breathing-circuit-systems
Verena Spertini1, Livia Borsoi, Jutta Berger
1Clinical Institute for Hospital Hygiene, Medical University of Vienna, Vienna, Austria.
Background:
Bacterial contamination of anesthesia breathing machines and their potential hazard for pulmonary infection and cross-infection among anesthetized patients has been an infection control issue since the 1950s. Disposable equipment and bacterial filters have been introduced to minimize this risk. However, the machines' internal breathing-circuit-system has been considered to be free of micro-organisms without providing adequate data supporting this view. The aim of the study was to investigate if any micro-organisms can be yielded from used internal machines' breathing-circuit-system. Based on such results objective reprocessing intervals could be defined.
Methods:
The internal parts of 40 anesthesia machines' breathing-circuit-system were investigated. Chi-square test and logistic regression analysis were performed. An on-site process observation of the re-processing sequence was conducted.
Results:
Bacterial growth was found in 17 of 40 machines (43%). No significant difference was ascertained between the contamination and the processing intervals. The most common contaminants retrieved were coagulase negative Staphylococci, aerobe spore forming bacteria and Micrococcus species. In one breathing-circuit-system, Escherichia coli, and in one further Staphylococcus aureus were yielded.
Conclusion:
Considering the availability of bacterial filters installed on the outlet of the breathing-circuit-systems, the type of bacteria retrieved and the on-site process observation, we conclude that the contamination found is best explained by a lack of adherence to hygienic measures during and after re-processing of the internal breathing-circuit-system. These results support an extension of the re-processing interval of the anesthesia apparatus longer than the manufacturer's recommendation of one week. However, the importance of adherence to standard hygienic measures during re-processing needs to be emphasized.
Insights
Bacterial contamination is common in anesthesia breathing circuits, with 43% of machines showing growth. Proper reprocessing and hygiene are crucial to prevent infections and may allow for longer intervals between cleaning anesthesia equipment.
Area of Science:
- Anesthesiology
- Infection Control
- Microbiology
Background:
- Bacterial contamination of anesthesia breathing machines poses a risk for pulmonary and cross-infections.
- While disposable equipment and filters exist, the internal breathing circuits are often assumed to be sterile.
- Data supporting the sterility of internal breathing circuits is lacking.
Purpose of the Study:
- To investigate microbial contamination within used anesthesia machine breathing-circuit systems.
- To gather data for defining objective reprocessing intervals for anesthesia equipment.
Main Methods:
- Investigated internal parts of 40 anesthesia machines' breathing-circuit systems.
- Utilized Chi-square test and logistic regression analysis.
- Conducted on-site observation of reprocessing procedures.
Main Results:
- Bacterial growth detected in 17 out of 40 (43%) investigated machines.
- No significant correlation found between contamination levels and reprocessing intervals.
- Common contaminants included coagulase-negative Staphylococci, spore-forming bacteria, and Micrococcus species; E. coli and S. aureus were found in isolated cases.
Conclusions:
- Contamination is primarily attributed to inadequate adherence to hygienic measures during reprocessing.
- Findings suggest that current manufacturer recommendations for weekly reprocessing may be extended.
- Emphasizes the critical importance of maintaining standard hygienic practices during the reprocessing of anesthesia apparatus.
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