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[Determination of longistylin A and longistylin C in Cajanus cajan]
Dawu Wang1, Minxun Xiao, Yirong Li
1Tropical Medicine Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China. dwwang66@163.com
Objective:
To establish quality control criteria for medicinal herb Cajanus cajan based on the determination of longistylin A and longistylin C, two bioactive and specific stilbenes of the plant.
Method:
Longistylin A and longistylin C were obtained from the leaves of C. cajan by silica gel column chromatography and identified as marker compounds of this plant by spectroscopic analysis. A RP-HPLC method was established to determine the two compounds.
Result:
Longistylin A and longistylin C were well separated on a Thermo BDS Hypersil C18 column (4.6 mm x 250 mm, 5 microm) with a mobile phase methanol-water (8:2), and showed good linearity in the range of 0.00288 - 0.0576 microg and 0.0112 - 0.224 microg, respectively. The average recoveries were 98.9% and 97.2% with RSD of 2.4% and 2.2% for these two compounds, respectively.
Conclusion:
The established analysis method is simple and accurate, whicn can be used for quality control of C. cajan.
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