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Published on: September 23, 2015
Enamel alterations in serotonin 2B receptor knockout mice
Yassine Harichane1, Sasha Dimitrova-Nakov, Arnaud Marchadier
1INSERM, UMR-S 747, F-75006, Paris, France.
European Journal of Oral Sciences
|January 17, 2012
Summary
The serotonin 2B receptor (5-HT(2B) R) is crucial for tooth enamel development. Knockout mice lacking this receptor showed significantly reduced enamel volume and structural defects, impacting mineralization.
Area of Science:
- Biochemistry
- Developmental Biology
- Dental Research
Background:
- The serotonin 2B receptor (5-HT(2B) R) is implicated in various physiological processes.
- Its specific role in tooth enamel formation and mineralization remains largely unexplored.
Purpose of the Study:
- To investigate the function of the 5-HT(2B) R in enamel formation and mineralization.
- To compare enamel characteristics in 5-HT(2B) R knockout (KO) mice with wild-type (WT) mice.
Main Methods:
- Micro-computed tomography (micro-CT) imaging for quantitative enamel volume analysis.
- Energy-dispersive X-ray microanalysis to assess mineralization.
- Scanning electron microscopy (SEM) to examine enamel microstructure.
Main Results:
- KO mice exhibited significantly reduced enamel volume in molars compared to WT mice.
- Defective mineralization and structural abnormalities, including altered Hunter-Schreger bands, were observed in KO mice.
- Minor disturbances were also noted in the incisors of KO mice.
Conclusions:
- The 5-HT(2B) R plays a significant role in regulating enamel formation and mineralization.
- Disruption of 5-HT(2B) R signaling impairs ameloblast function and amelogenesis.
- These findings highlight the importance of 5-HT(2B) R in maintaining healthy tooth enamel structure.
