Related Experiment Video
Updated: May 25, 2026

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles
Published on: July 11, 2025
Spider: an R package for the analysis of species identity and evolution, with particular reference to DNA barcoding
Samuel D J Brown1, Rupert A Collins, Stephane Boyer
1Bio-Protection Research Centre, PO Box 84, Lincoln University 7647, Canterbury, New Zealand. sam.brown@lincoln.ac.nz
Abstract:
Spider: SPecies IDentity and Evolution in R is a new R package implementing a number of useful analyses for DNA barcoding studies and associated research into species delimitation and speciation. Included are functions essential for generating important summary statistics from DNA barcode data, assessing specimen identification efficacy, and for testing and optimizing divergence threshold limits. In terms of investigating evolutionary and taxonomic questions, techniques for assessing diagnostic nucleotides and probability of reciprocal monophyly are also provided. Additionally, a sliding window function offers opportunities to analyse information across a gene, essential for marker design in degraded DNA studies. Spider capitalizes on R's extensible ethos and offers an integrated platform ideal for the analysis of both nucleotide and morphological data. The program can be obtained from the comprehensive R archive network (CRAN, http://cran.r-project.org) and from the R-Forge package development site (http://spider.r-forge.r-project.org/).
Related Concept Videos
Evolutionary Relationships through Genome Comparisons
Modern Molecular Taxonomy
Applications of Molecular Taxonomy
Phylogenetic Species Concept in Microbiology
Statistical Package for the Social Sciences (SPSS)
SPSS streamlines the process from data preparation to analysis and reporting. It is characterized by its user-friendly interface, which conceals...
Methods of Classification and Identification

